D. Assan, X. Gao, J.C.K. Lai, S.W. Leung
Idaho State University, US
oxidation, nitrite, peroxide, nanogold particles, biosensor
Hemoglobin has a very strong affinity with oxygen and thus is a good catalyst to carry oxygen for many oxidation reactions. Many researchers have explored this property of hemoglobin to develop sensors for the detection of nitrite and peroxide, which are important in biomedical and environmental monitoring. We have previously reported the development of biosensors that can detect nitrite and peroxide with a lower detecting limit of much lower than 1x10-12 M, that were among the best detectors at the time. With a similar sensor platform, we have now developed a biosensor that can detect the oxidation reaction orders of magnitude better than the previous sensors. The sensor comprises layers of biocomposite made up of a polymer, nano Au particles, and a complex protein on the surface of an electrode. The performance of this electrode biosensor will be compared with the previously developed sensors, the durability and other factors that can affect the performance and fabrication of this biosensor are discussed.