Photoreduction of NAD(P) by activated dye molecule-DNA monolayer
S. Nokhrin, S. Gaballah, J.S. Lee
University of Saskatchewan, CA
Photovoltaics, Hydrogen, NAD(P)H, dye molecule
Photoelectrolytic cells for converting optical energy into chemical energy have attracted much attention as promising elements for alternative renewable and non-polluting energy sources. These devices can transform light energy (potentially, sunlight) into electrical energy in photovoltaic cells or into chemical energy that can be stored in the form of hydrogen or other chemical product. In our work we have studied possibility of photo-induced production of NAD(P)H at dye molecule-DNA monolayer. In our cell gold electrode incubated with a fluorescein (Fl)-labeled DNA monolayer is immersed into solution of NAD(P)+. Under incident irradiation (wavelength 473 nm) and small applied potential a photocurrent was generated that ultimately reduced NAD(P)+ to NAD(P)H. While in direct electrochemical reduction experiments the NAD-dimers are mainly produced at negative potential and NAD(P)H can be produced only at very negative potentials, in our photoelectrolytic cells under light irradiation NAD(P)+ was reduced to NAD(P)H under much smaller potentials. The mechanism of photoinduced NAD(P)H production in photoelectrolytic cell is discussed.
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